Digestiflow User Manual

DigestiFlow is a suite of programs for managing Illumina flow cells, curating sample sheets, and demultiplexing. Digestiflow Server is the database system offering a web-based user UI and REST API.


The Digestiflow Server documentation is currently under construction and in beta state. Don’t hesitate to open issues in our GitHub bug tracker in case of any question or problem.

Citing DigestiFlow

Please cite DigestiFlow as:

Holtgrewe, Manuel, Clemens Messerschmidt, Mikko Nieminen, and Dieter Beule. “DigestiFlow: From BCL to FASTQ with Ease.” Bioinformatics, November 14, 2019, btz850. https://doi.org/10.1093/bioinformatics/btz850.